RNF219 regulates CCR4-NOT function in mRNA translation and deadenylation

RNF219 regulates CCR4-NOT function in mRNA translation and deadenylation

Blog Article

Abstract Post-transcriptional regulatory mechanisms play a role in many biological contexts through the control of mRNA degradation, translation and localization.Here, we show that the SHIRTS CAREER REGULAR FIT RING finger protein RNF219 co-purifies with the CCR4-NOT complex, the major mRNA deadenylase in eukaryotes, which mediates translational repression in both a deadenylase activity-dependent and -independent manner.Strikingly, RNF219 both inhibits the deadenylase activity of CCR4-NOT and enhances its capacity to Chlorella repress translation of a target mRNA.We propose that the interaction of RNF219 with the CCR4-NOT complex directs the translational repressive activity of CCR4-NOT to a deadenylation-independent mechanism.